%0 Journal Article %A sheibani, shahin %A daryanoosh, farhad %A salesi, mohsen %A koushkie jahromi, maryam %A tanideh, nader %T The effect of high-intensity training and detraining on FOXO3a/MuRF1 and MAFbx levels in soleus muscle of male rats %J EBNESINA %V 20 %N 1 %U http://ebnesina.ajaums.ac.ir/article-1-570-en.html %R %D 2018 %K Physical Activity, Atrophy, Hypertrophy, Rat, %X Background Inactivity may result in loss of muscle volume and subsequently reduce the power, by activating proteasome signaling pathways, which leads to a decrease in quality and expectations of life. The aim of this study was to evaluate the effect of high-intensity training and also detraining on the signaling pathways of FOXO3a, MAFbx, and MuRF1. Materials and methods: In this study, 32 2-month male Sprague-Dawley rats were used. At first, periodic exercise with intensity of 85-100% VO2max was performed for six weeks. Then, the groups (detrained for 48h, 7w, and 14w) were detrained and then soleus muscle was removed from hind limb. H&E staining and histochemical procedure were used to measure the amount and percentage of skeletal muscle fibers type. Also, RT-PCR method was used to examine gene changes. Results: The mean weight of the soleus muscle increased after exercise and decreased significantly after detraining (p=0.001).The fiber type conversion were happened from I to IIA (p=0.001). Hypertrophy was observed in fibers after training, whereas atrophy was seen after detraining in type II fibers (p=0.001). FOXO3a and MAFbx levels were significantly increased after training and decreased after detraining period (p=0.001).The MuRF1 expression shown an increase and a decrease after training and detraining, respectively (p=0.001). Conclusion: The results of this study indicated that the exercise couldn’t prevent atrophy during the period of detraining. Also, the activation of pathway FOXO3a/MAFbx, not the FOXO3a/ MuRF1, is the atrophy inducer during detraining. In this regard, probably MuRF1 is an indirect target of FOXO3a and cannot always be an appropriate marker of atrophy. MuRF1 probably plays a more important role in pathological atrophy conditions. %> http://ebnesina.ajaums.ac.ir/article-1-570-en.pdf %P 31-39 %& 31 %! %9 Original %L A-10-443-2 %+ %G eng %@ 1735-9503 %[ 2018