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:: Volume 20, Issue 1 (spring 2018) ::
EBNESINA 2018, 20(1): 31-39 Back to browse issues page
The effect of high-intensity training and detraining on FOXO3a/MuRF1 and MAFbx levels in soleus muscle of male rats
Shahin Sheibani, Farhad Daryanoosh , Mohsen Salesi, Maryam Koushkie jahromi, Nader Tanideh
, daryanoosh@shirazu.ac.ir
Abstract:   (1495 Views)
Background Inactivity may result in loss of muscle volume and subsequently reduce the power, by activating proteasome signaling pathways, which leads to a decrease in quality and expectations of life. The aim of this study was to evaluate the effect of high-intensity training and also detraining on the signaling pathways of FOXO3a, MAFbx, and MuRF1.
Materials and methods: In this study, 32 2-month male Sprague-Dawley rats were used. At first, periodic exercise with intensity of 85-100% VO2max was performed for six weeks. Then, the groups (detrained for 48h, 7w, and 14w) were detrained and then soleus muscle was removed from hind limb. H&E staining and histochemical procedure were used to measure the amount and percentage of skeletal muscle fibers type. Also, RT-PCR method was used to examine gene changes.
Results: The mean weight of the soleus muscle increased after exercise and decreased significantly after detraining (p=0.001).The fiber type conversion were happened from I to IIA (p=0.001). Hypertrophy was observed in fibers after training, whereas atrophy was seen after detraining in type II fibers (p=0.001). FOXO3a and MAFbx levels were significantly increased after training and decreased after detraining period (p=0.001).The MuRF1 expression shown an increase and a decrease after training and detraining, respectively (p=0.001).
Conclusion: The results of this study indicated that the exercise couldn’t prevent atrophy during the period of detraining. Also, the activation of pathway FOXO3a/MAFbx, not the FOXO3a/ MuRF1, is the atrophy inducer during detraining. In this regard, probably MuRF1 is an indirect target of FOXO3a and cannot always be an appropriate marker of atrophy. MuRF1 probably plays a more important role in pathological atrophy conditions.
Keywords: Physical Activity, Atrophy, Hypertrophy, Rat
Full-Text [PDF 804 kb]   (621 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/09/15 | Published: 2018/04/15
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sheibani S, daryanoosh F, salesi M, koushkie jahromi M, tanideh N. The effect of high-intensity training and detraining on FOXO3a/MuRF1 and MAFbx levels in soleus muscle of male rats. EBNESINA. 2018; 20 (1) :31-39
URL: http://ebnesina.ajaums.ac.ir/article-1-570-en.html

Volume 20, Issue 1 (spring 2018) Back to browse issues page
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